The aim of the study was to test the effect of the mineral salt composition in the medium, concentration of plant growth regulators and the medium pH on the in vitro multiplication and shoot growth of C.
The smoke bush (Cotinus coggygria Scop.) is a popular and highly demanded ornamental shrub whose propagation can be problematic. All of the RAPD and ISSR profiles were monomorphic and comparable with the mother plant. A total of 30 random amplified polymor-phic DNA (RAPD) and 20 inter-simple sequence repeat (ISSR) primers resulted in 197–199 and 184–187 distinct and reproducible band classes, respectively, in 'Aurea' and 'Elegantissima' plantlets. Genetic stability of the micropropagated plants was confirmed by using two DNA-based molecular marker techniques. The final survival rate of the plants after 20 wk was 80% for 'Aurea' and 90% for 'Elegantissima'. Microcuttings were rooted for 8 wk on medium enriched with 0.25 mg L −1 NAA and potted into P9 containers in the greenhouse. Rooting of regenerated shoots was achieved by an in vitro method when different concentrations of NAA or indole-3-butyric acid (IBA) were tested. Regardless of the cultivar, the best shoot proliferation was observed on Lloyd and McCown medium (woody plant medium (WPM)) at pH 6.2, containing 1.0 mg L −1 BA, 0.1 mg L −1 NAA, and 20–30 g L −1 sucrose.
Media were supplemented with either N 6-benzyladenine (BA) or thidiazuron (TDZ) in combination with 1-naphthaleneacetic acid (NAA). alba cultivars 'Aurea' and 'Elegantissima' were cultured on media enriched with six different sources of mac-ronutrients. The vegeta-tive propagation of dogwood by cuttings may be slow, difficult, and cultivar dependent therefore, an improved micropropagation method was developed. (white dogwood) is an important ornamental shrub having a wide range of applications such as reforestation programs and soil retention systems.
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